骨髓增生异常综合征患者间充质干细胞SDF-1基因的表达

【摘要】  本研究探讨骨髓基质细胞衍生因子-1(SDF-1)在骨髓增生异常综合征（MDS）患者骨髓间充质干细胞（MSC）中的表达水平。采集MDS的骨髓标本，分离、培养和扩增MSC，观察细胞形态并进行表型鉴定；以实时定量RT-PCR法检测间充质干细胞SDF-1基因和内参照磷酸甘油醛脱氢酶（GAPDH）基因的表达水平，并与健康供者的表达水平相比较。结果发现，MDS患者骨髓间充质干细胞SDF-1基因的表达水平经GAPDH校正后为1.53±0.92，与对照组(5.51±0.99)相比差异非常显著(P<0.01)。结论：SDF-1基因在MDS患者骨髓间充质干细胞中的表达显著升高，SDF-1基因的异常表达可能影响MDS患者骨髓微环境的造血调控作用，是否可对MDS的发病机制及治疗提供新的思路值得进一步探讨。

【关键词】  骨髓增生异常综合征

　　Expression of SDF-1 Gene in Bone Marrow Mesenchymal Stem Cells of  Patients with   Myelodysplastic Syndrome

　　Abstract  This  study was aimed  to  investigate the expression level of stromal cell derived factor-1 gene (SDF-1) in bone marrow mesenchymal stem cells (MSC) of patients with myelodysplastic syndrome (MDS).The MSC from bone marrow samples of MDS patients were isolated，cultured and expanded，the morphology and immunophenotype of MSC were analyzed.The expression levels of SDF-1 and internal reference GAPDH in MSC of MDS patients were detected by  real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR)  method  and were  compared with expression levels of healthy donors.  The results showed that the expression levels of SDF-1 in  MDS patients were significantly different from those in healthy donors    (1.53±0.92 vs 5.51±0.99) (P<0.01).  SDF-1 gene expression levels in bone marrow MSC of  MDS patients were significantly higher than that in MSC derived from healthy donors.It

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