干扰素α2b对HL60细胞抑增殖与促凋亡的影响

作者：硕博网文章来源：中华硕博网点击数：更新时间：2008-5-3

中华硕博网(http://www.china-b.com)全球500所高校指定报名中心--干扰素α2b对HL60细胞抑增殖与促凋亡的影响

【摘要】 为了研究干扰素α2b（IFNα2b）对HL60细胞增殖与凋亡的影响，采用瑞氏染色观察HL60细胞的形态变化，吖啶橙/溴化乙锭双染色观察细胞凋亡，通过CD13测定观察细胞膜抗原表达及成熟分化，MTT实验观察增殖活性。结果显示：加入IFNα2b 48小时后，HL60细胞凋亡率升高，增殖活性下降，而且随着IFNα2b浓度的升高，抑制作用更加明显。CD13表达逐渐下降，细胞形态逐渐向成熟转化。结论：IFNα2b 能促进HL60细胞凋亡、抑制HL60细胞增殖和促进分化成熟。

【关键词】 干扰素干扰素α2b HL60细胞细胞凋亡细胞增殖

Influence of Interferonα2b on Proliferation Inhibition and Apoptosis Induction in HL60 Cells

Abstract To investigate the effects of interferonα2b on proliferation and apoptosis in HL60 cells, HL60 cells were cultured in different concentrations of IFNα2b. The morphologic changes were observed by Wright′ s and acridine orange (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation was detected by MTT. Expression of CD13+ was checked by indirect fluoroimmunoassay. The results showed that apoptosis rate of HL60 cells assayed by the abovementioned two methods was (51±2)% and (78±3)% respectively and OD570 values of proliferation inhibited were 1.8±0.1 and 1.0±0.1 respectively when the concentrations of the IFNα2b were 500 and 10 000 U/ml in culture for 48 hours. Morphology and count of CD13+ cells were changed. CD13+ cell expression rate was (62±2)% and (30±3)% respectively when the concentrations of the IFNα2b were 500 and 10 000 U/ml in culture for 48 hours.It is concluded that IFNα2b can enhance the apop

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