VEGF基因特异性siRNA转染后对人乳腺癌细胞增殖和凋亡的影响

               作者：张晓, 徐文华, 葛银林, 侯琳, 李泉

【关键词】  RNA干扰;,小干扰RNA;,MCF

　　Effect of siRNA transfection targeting VEGF gene on proliferation and apoptosis of human breast cancer cells

　　[Abstract]  AIM: To explore the effect of RNA interferencemediated vascular endothelial growth factor (VEGF) gene silencing on proliferation and apoptosis of human breast cancer MCF7 cells. METHODS: Small interfering RNA (siRNA) targeting VEGF gene was designed and DNA template was synthesized, then siRNA was obtained by in vitro transcription. After dssiRNA was transfected into MCF7 cells with Lipofectamine, the proliferation of MCF7 cells was detected by MTT colorimetry, and the apoptosis was measured by Hoechst33258 staining. The change of cell cycle was analyzed by flow cytometry (FCM). The expression of VEGF on mRNA and protein level was analyzed by RTPCR and immunohistochemical technology respectively. RESULTS: The siRNA targeting human VEGF gene effectively inhibited the proliferation of MCF7 cells, induced cell apoptosis, arrested the cell cycle in G0/G1 phase, and decreased the expression of VEGF on both mRNA and protein level. But these effects did not appear in scrambled siRNA (siRNASCR) control group. CONCLUSION: The siRNA targeting human VEGF gene could reduce VEGF expression, inhibit cellular proliferation and induce apoptosis in MCF7 cells in vitro.

　　[Keywords]RNA interference; siRNA; MCF7 cell; VEGF; gene therapy

[1] [2] [3] 下一页