ST6Gal I 基因特异性siRNA对SW480细胞黏附和侵袭力的影响

                    作者：苑天红, 李明远, 李婉宜, 李虹, 蒋忠华

【关键词】  siRNA;,结肠肿瘤;,唾液酸转移酶;,黏附;,肿瘤侵袭力

　　EffectofST6GalIsiRNAmediatedgene silencing on the adhesion and invasion of SW480 cells

　　[Abstract]AIM: To study the effect of synthesized ST6Gal I specific siRNA on the adhesion and invasiveness of human colon carcinoma cell line SW480 with over expression of ST6Gal I. METHODS: A double strand small interference RNA (siRNA) targeting ST6Gal I was designed and synthesized, and then transfected into SW480 cells by lipofectmine 2000. SW480 cells were cultured and divided into 4 groups: blank control group, liposome control group, nonspecific siRNA group and ST6Gal I siRNA group. The expression of ST6Gal I mRNA was examined by RTPCR and the amount of α2,6sialylation on the SW480 cell surface was detected by flow cytometry. The adhesion and invasion of SW480 cells to extracellular matrix (ECM) were analyzed by using CytoMatrixTM kit and cell invasion assay kit, respectively. RESULTS: After SW480 cells were transfected for 48 hours, the expression of ST6Gal I mRNA in ST6Gal I siRNA group was significantly decreased compared with that in the blank control group, liposome control group, and nonspecific siRNA group (P<0.05). After SW480 cells were transfected  for 72 hours, the amount of α2,6sialylation on cell surface, the adhesion and invasion of the cells in ST6Gal I siRNA group were markedly lower than those

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