血小板第4因子对急性放射损伤小鼠骨髓基质细胞的保护作用

        作者：高瑛，杨岚，田琼，方恒虎，聂蕾，刘水冰，卫张蕊

【关键词】  骨髓基质细胞

    Protective effects of platelet factor 4  on bone marrow stromal cells of acute radiation injury in mice

　　【Abstract】 AIM:  To investigate the protective effects of platelet factor 4 (PF4) on bone marrow stromal cells (BMSCs) of radiated mice and to study the protective mechanism of hematopoietic radiation injury in mice. METHODS:  Forty male mice were randomized to four groups (R, R+P, N+P and N) and two of groups (R, R+P) were exposed wholebodily to 5.0 Gy 60Co γrays. The mice were treated with injections of PF4 (50μg/ kg), ip twice at an interval of 6 h and 20 h. After the second injection, they were irradiated. The cell cycle and apoptosis of bone marrow stromal cells (BMSCs) cultured in vitro after radiation were analyzed by FACS after ten days culture. The expression of p27kip1 in the cells was detected using immunohistochemistry. RESULTS:  The 24th hour adhesion rate of BMSC in radiated mice respectively reached 37%, 58%, 74% and 79.3%. The cell percentage of G0+G1 phase increased and those of G2+M decreased in R group, compared with those in R+P, N+P and N groups. But the DNA content of the radiated group of BMSC decreased remarkably compared with those in the other three groups. The expression of p27kip1 was downregulated compared with that of BMSC without PF4 protection. CONCLUSION:  The protective effects of PF4 on BMSCs with radiated injury probably result from the inhibiti

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