太子参醇提物对大鼠组织和红细胞的抗氧化活性

【关键词】  太子参醇提物

    Antioxidative activity of EPHPH on tissues and erythrocytes of rats

　　【Abstract】 AIM: To study the antioxidative activity of the alcoholic extract from Pseudostellaria heterophylla Pax et Hoffm (EPHPH) and its mechanism. METHODS:  The peroxidation in homogenates of heart and liver and kidney of rats was induced by ・HO generation system Fe2++ascorbic acid. Malondialdehyde（MDA）was determined using TBA colorimetric method and superoxide（O2-）from zymosanstimulated neutrophils of rats was measured by NBT reduction method. H2O2caused hemolysis of erythrocytes was measured spectrometrically. RESULTS:  MDA formation in homogenates of heart and liver and kidney of rats induced by ・HO generation system Fe2++ascorbic acid was inhibited differently by 6.7, 13.4, 26.8, 53.5, 107.0 and 214.0 μg/L EPHPH, whose IC50 that inhibited MDA formation in heart, liver and kidney was 57.0, 51.5  and 53.2 μg/L respectively, whose relation between quantity and efficiency showed all negative  and whose relative coefficient was -0.891,-0.883 and -0.893 respectively (all P<0.001). O2-  generation from zymosanstimulated neutrophils of rats was inhibited distinctly by EPHPH, whose IC50 that inhibited O2- generation was 83.1 μg/L, whose relation between quantity and efficiency showed negative  and whose relative coefficient wa

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