白藜芦醇对豚鼠心室肌细胞L

            作者：易方方，唐其柱，冯斯婷，王腾

【关键词】  白藜芦醇；全细胞膜片钳；钙电流

　　Effects of resveratrol on Ltype calcium current in isolated ventricular myocytes of guinea pig

　　【Abstract】 AIM: To investigate the effects of resveratrol on the Ltype calcium current (ICaL) in isolated ventricular myocytes of  guinea pig. METHODS: Single ventricular cell was obtained from healthy guinea pig heart using enzymatic dissociation methods. The wholecell patch clamp was used to record the ICaL following the administration of resveratrol at 1, 50, 100 μmol/L. RESULTS: ① Resveratrol reduced the ICaL in a concentrationdependent manner, but it had no effect on IV shape of ICaL. ② Resveratrol markedly shifted the steadystate activation curve of ICaL to the right,  and V1/2 value increased from (-18.97±4.08) mV in control to (-15.97±4.13) mV in the presence of resveratrol （n=7, P=0.02）, the κ value from (3.64±0.56) to (3.82±0.60) (n=7，P=0.67). ③ Inactivation curve of ICaL was not affected significantly. ④ The time constant of reactivation of ICaL  was reduced from (98.50±9.00) ms to (54.53±4.27) ms (n=6，P=0.000) by resveratrol. CONCLUSION: Resveratrol inhibited the Ltype calcium channel, mainly acting on the activity and recovery processes.

　　【Keywords】 resveratrol；wholecell patch clamp；calcium current

　　【摘要】 目的：研究白藜芦醇对正常豚鼠心室肌细胞L型钙电流（ICaL）的影响. 方法：采用全细胞膜片钳技术,记录不同浓度白藜芦醇作用前后对ICaL的影响. 结果：①白藜芦醇呈浓度依赖性阻断ICaL,但对激活电位、峰电位、反转电位均没有影响；②白藜芦醇使ICaL稳态激活曲线右移，V1/2由对照的（-18.97±4.08）

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